Where absorbance = epsilon (wavelength dependent constant)*path length * concentration[4]. With the definition alone it is clear that the Beer-Lambert Law is evident in the use of the calibration curve where the axis are the same. However, with the law there comes some limitations. One main limitation is the scattering of light due to particles or their being bubbles within the cuvette, what the particles cause is the light to not go directly through the sample. The initial intensity would be much higher than the intensity of the light through the sample. It would be similar for air bubbles, the light would go through the bubbles and possibly reflect causing the readings to be
Where absorbance = epsilon (wavelength dependent constant)*path length * concentration[4]. With the definition alone it is clear that the Beer-Lambert Law is evident in the use of the calibration curve where the axis are the same. However, with the law there comes some limitations. One main limitation is the scattering of light due to particles or their being bubbles within the cuvette, what the particles cause is the light to not go directly through the sample. The initial intensity would be much higher than the intensity of the light through the sample. It would be similar for air bubbles, the light would go through the bubbles and possibly reflect causing the readings to be