* For the first one this is what I would do... okay so we have the original equation for the first one & based off that this is what I would do... I would discover f ( 2 ) by inserting x equals 2. f…
Lab #5: Introduction to Metasploit on Kali Linux Team: CRYPTERS 1 d. Why is it usually a bad idea to operate in the Linux environment as root? If you are unfamiliar with the concept of the root user, do a quick google search. It is always a good practice on any operating system to run your applications on a user level and leave the administrative tasks to the root user, and only on a per-need basis. Applications are meant to be run by users with non-administrative privileges.(Power December 4, 2010)…
A sketch or view of test setup and arrangement of gauges and loading protocol should be presented. It is not clear the location of gauge 1 and 2 listed in Figure 11. Why the results of two mentioned gauges are presented in comparison with analytical one? Are the both of them comparable? Why the results of two gauges are different?…
After approval from the professor, the first part of the unknown was to isolate all the gram positive bacteria since the unknown…
Materials. Numerous substances in the experiment were used. The most frequently used was the unknown due to the need to test its physical and chemical qualities. When a solution of the unknown was made, 1.000 g of the unknown and 1.0 mL of water was used to make it. To test for the possible ions, 1.0 mL of silver nitrate and 1.0 mL of nitric acid were used for the ion test.…
The study of microbiology requires not only understanding the microscopic organisms, but also the understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. The identification of microorganisms is not only important in microbiology lab, but also in the medical field to identify an agent of a disease that will help treat the patient by using the correct antibiotics to kill off the host. In this unknown lab report, techniques and procedures learned in the microbiology laboratory during the semester that was performed to test ones practical understanding of microbiology. The sole purpose of the unknown lab is to demonstrate understanding of the experimental methods and lab techniques learned during…
ABSTRACT Working with infectious agents that require BSL-3 level containment agents offers many challenges for researchers. BSL-3 containment laboratories are usually not equipped with expensive specialty equipment that is needed for studies such as flow cytometric analysis, microscopy, and proteomic analyses. Therefore, for most researchers that are working with BSL-3 level infectious agents, removal of samples from BSL-3 labs for these types of studies is necessary, and methods for complete and dependable inactivation of the samples are required. In this report we have done a thorough characterization of the effectiveness of paraformaldehyde fixation for inactivation of cell samples infected with the intracellular bacterial agents Burkholderia pseudomallei (Bp) and Francisella tularensis (Ft), both of which are Tier 1 select agent pathogens that require BSL-3 containment. We have demonstrated that cells infected with these pathogens are completely inactivated via 5-minute treatment with 4% paraformaldehyde.…
Three agar plates were labeled, each containing the type of treatment, TA name, class section and group number. With a sterile pipet 130 µL mixed bacterial suspension were removed from the tube labeled “C” and the lid from the control was removed, as well as the bacteria dispensed onto the agar. A cell spreader was dipped in ethanol and was used to spread the bacteria evenly along the agar surface. The transfer of 130 µL of bacterial suspensions from the “lux” tube was placed into the “lux” plate and the cells were spread onto the agar surface once again. The cells in the “NP” tubes were plated onto 2 plates labeled “NP” and the lids were replaced with new ones; the plates were left out in room temperature so the liquid could be absorbed within 10 minutes.…
The focus of this lab was to identify an unknown organism based on its characteristics and the results from each of the tests. There will be various of test to choose from in order to identify the unknown organism, which will eliminate numerous possibilities and narrow it down to one. All the fundamental skills that we have learned and practiced in the lab will be used to perform on our unknown such as aseptic technique, microscopic examination, the use of differential media, and determining if it’s positive or negative. Performing aseptic techniques is the most crucial step that requires the utilizing of transferring, inoculating, and storing bacterial cultures and media. Aseptic technique is defined as procedures that prevent contamination…
Yes there was growth in the agar plates however there was not as much growth as predicted. Another problem that arose was the fact that the pGLO did not attach to any Escherichia coli DNA, so when under a UV light, none of the agar plates glowed, even the ones that were supposed to. After observing and recording the results that were collected, it can be concluded that due to human…
Fat is a Drosophila tumour suppressor gene that regulates the growth of cells in tissues. The McNeill lab are trying to understand how Fat restricts growth and controls Hippo pathway activity. This is being tested using flies and mice. They are also investigating how Fat regulates growth via control of metabolism and mitochondrial function. The McNeill lab is hoping to find how Fat regulates the growth rate and full grown size of organs.…
Both tubes were labeled, one being positive pGLO, and the other negative pGLO. Then, using a long sterile loop, each group picked up a single colony of E. coli that was then mixed throughout the positive tube and returned back to the ice. The same was then done for the negative pGLO tube. With a new sterile loop, each lab group obtained a sample of pGLO plasmid DNA and submerged it into only the positive tube and placed it back into the ice. Each group then received four Luria Bertani broth nutrient agar plates and labeled them +pGLO LB/amp, +pGLO LB/amp/ara, -pGLO LB/amp, and –pGLO LB, the amp standing for ampicillin and the ara for arabinose.…
Throughout this experiment I have learned several things. One thing I learned is that you can find bacterial growth in most places. However, different types of bacteria grow in different places depending on the environmental conditions and the required factors for growth for the specific bacteria. Also, I learned is the impact an error in the procedure can have. I think there were some errors in our environmental sampling lab.…
The bacteria grew because there was a source of food present to help the bacteria grow and reproduce and there was beta lactamase in the DNA plasmid which allowed the cell to become resistant to ampicillin. It ended up glowing after the procedure because the arabinose allowed the green fluorescent protein in the DNA plasmid to be expressed. The araC gene that repressed the green fluorescent protein became repressed by arabinose. The hypothesis about the -pGLO plate containing LB and ampicillin was correct because the bacteria did not grow or glow after the procedure. It did not grow because there was ampicillin present on the plate, which interfered with the cell’s ability to synthesize the cell’s walls and made cell growth impossible.…
The lab instructor issued out a test tube labeled with letter ‘I’, which consisted of two unknown bacteria, Gram-positive or Gram-negative that were streaked from a pure culture. Sterile techniques were followed while performing precise instructions as stated in the referenced Laboratory Manual. Example 1: The first procedure performed was done by isolating a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it turned red to ensure that there were no current bacteria on the loop avoiding contamination followed by rapidly flaming the neck of the test tube to prevent the entry and contamination of unwanted microbes.…