Data was collected from groups who acquired …show more content…
The pH level of 8 showed an average absorbance of 0.167±0.030. The sources of variability consist of instrumental and operator errors. Instrumental errors include an inconsistent spectrophotometer and poorly calibrated micropipettes. Operator errors include unknowingly allowing trypsin to decay in an environment of room temperature, as well as the short length of incubation when mixing the trypsin, azocasein, and the reaction buffer. The potential sources that proved the most problematic include the condition of trypsin and the length of incubation. Trypsin must be kept in a cold environment when not in use, and the incubation of the samples must be long enough for the proteolysis to take place in the constant condition of 37°C for each trial. It is clear that in each trial for the pH 8, the extent of the breakdown of azeocasein