To determine the concentration of the red and blue dye, a concentration curve for each dye was created by using …show more content…
It was changed into a linear line graph. From the graph an equation was created, y = mx + b. The equation, y = mx + b was the same thing as A=Ɛℓc. The equation for the blue dye is .187 abs. = 102739x + 0 and the equations for the red dye is .244 abs. = 30260x + 0. These values were inputted into the beer’s law, A=Ɛℓc (A = y, Ɛℓ= M, c= x). To find concentration, beer’s law was rearranged to AƐℓ=c. The final concentration of the red dye was 1.82x10-6 and the blue dye was 8.06x10-6.
Let alone, the final concentration was found, then the final volume can be found. The equation M1V1=M2V2 must be change into (M_1 V_1)/M_2 =V_2. The final volume needed for the red dye was 5.00 mL, the blue dye 3.60 mL, and the rest was filled up with deionized water up to 25 mL in a volumetric flask. Only one trial was done. To measure the accuracy of the created solution, the solution was put in a UV-Vis spectrometer. Then the absorbance curve of the created solution was compared to the absorbance curve of the Gatorade.
Discussion/Results
Food dyes that was presented in the low-calorie Gatorade was FD&C Blue #1 and FD&C Red #40. The UV-Vis spectrometer showed that wavelengths 514 nm and 629.6 nm had absorbance peak. Using a color spectrum, the wavelengths corresponds to green and orange. The colors that were emitted were red and blue. Figure 1: The absorbance curve of the low-calorie