After the agar plate was divided, they were then labeled “E. Coli + fresh/dry specific spice. Each of the eight agar plates were labeled this was and then ¼ of the agar plate was labeled control. This is where the plain 7mm disc went and this acted as the control of the experiment. Before any of the discs were put on the agar plate, the agar plate was prepared by making a bacterial lawn. In order to do this, a cotton swab was dipped into a tube containing E. Coli and then stirred around to insure that the bacteria was evenly distributed. Then the cotton swab was used to swab the entire surface of the agar plate. After one round of swabbing, the agar was rotated slightly to the right and swabbed again. This occurred one more time to ensure that the whole lawn would have bacteria
After the agar plate was divided, they were then labeled “E. Coli + fresh/dry specific spice. Each of the eight agar plates were labeled this was and then ¼ of the agar plate was labeled control. This is where the plain 7mm disc went and this acted as the control of the experiment. Before any of the discs were put on the agar plate, the agar plate was prepared by making a bacterial lawn. In order to do this, a cotton swab was dipped into a tube containing E. Coli and then stirred around to insure that the bacteria was evenly distributed. Then the cotton swab was used to swab the entire surface of the agar plate. After one round of swabbing, the agar was rotated slightly to the right and swabbed again. This occurred one more time to ensure that the whole lawn would have bacteria