To make a 12.5% resolving gel, a mixture of 30% acrylamide, 0.8% bisacrylamide, 4X Tris HCl buffer with pH 8.8, DI water, 10% ammonium persulfate, and TEMED. The solution was poured into a gel caster, ethanol was added on top, and it was left alone to polymerize. A 4.5% SDS stacking gel was then prepared by mixing: 30% acrylamide, 0.8% bisacrylamide, 4X Tris HCl buffer with SDS pH 6.8, DI water, 0.25% bromophenol blue, 10% ammonium persulfate, and TEMED. The gel was left to polymerize and the running buffer was prepared similar to the previous step. Following this the protein samples were prepared by calculating the volume of protein needed to add to each sample, by taking the amount of protein given and dividing by the protein concentration found on the table completed in experiment seven (Table 1).…